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1 kb dna ladder  (TaKaRa)


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    TaKaRa 1 kb dna ladder
    Validation of recombinant plasmids by restriction enzyme analysis and functional selection screening. (A) Restriction enzyme digestion of intermediate recombinant plasmids was performed using XhoI, XbaI , and KpnI . The observed banding patterns were consistent with the predictions generated by SnapGene software. Lanes 1–3 show the predicted digestion patterns of p15A-CmR-D90, p15A-CmR-D90-Δ5a/AmpR-ccdB, and p15A-CmR-D90-Δ5a/EGFP. Lanes 4–5, 6–7, and 8–9 correspond to the experimental digestion results of these respective plasmids. M: TaKaRa <t>1</t> <t>kb</t> DNA Ladder. All digested products were resolved by electrophoresis on 1% agarose gels and visualized with ethidium bromide staining. (B) Functional verification of the selection marker gene. The intermediate recombinant plasmid p15A-CmR-D90-Δ5a/AmpR-ccdB was transformed into two E. coli strains: GBred (CcdB-sensitive) and GBred- gyrA462 (CcdB-resistant). On LB agar plates containing ampicillin and chloramphenicol, only GBred- gyrA462 survived, confirming the toxicity of CcdB and functionality of the selection cassette. In the second recombination step, replacement of the AmpR-ccdB cassette by the target Δ5a/EGFP fragment yielded the final recombinant plasmid p15A-CmR-D90-Δ5a/EGFP. Upon counter-selection with CcdB, only host E. coli GBred harboring the correctly recombined plasmid survived on chloramphenicol-containing LB plates, while non-recombinant bacteria were eliminated.
    1 Kb Dna Ladder, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1+kb+dna+ladder/pmc13090652-136-2-1?v=TaKaRa
    Average 96 stars, based on 117 article reviews
    1 kb dna ladder - by Bioz Stars, 2026-08
    96/100 stars

    Images

    1) Product Images from "Construction and modification of a low-copy plasmid-based infectious clone for GI-19 genotype IBV via Red/ET recombineering: A simplified and efficient reverse genetics system for co ronavirus"

    Article Title: Construction and modification of a low-copy plasmid-based infectious clone for GI-19 genotype IBV via Red/ET recombineering: A simplified and efficient reverse genetics system for co ronavirus

    Journal: Poultry Science

    doi: 10.1016/j.psj.2026.106881

    Validation of recombinant plasmids by restriction enzyme analysis and functional selection screening. (A) Restriction enzyme digestion of intermediate recombinant plasmids was performed using XhoI, XbaI , and KpnI . The observed banding patterns were consistent with the predictions generated by SnapGene software. Lanes 1–3 show the predicted digestion patterns of p15A-CmR-D90, p15A-CmR-D90-Δ5a/AmpR-ccdB, and p15A-CmR-D90-Δ5a/EGFP. Lanes 4–5, 6–7, and 8–9 correspond to the experimental digestion results of these respective plasmids. M: TaKaRa 1 kb DNA Ladder. All digested products were resolved by electrophoresis on 1% agarose gels and visualized with ethidium bromide staining. (B) Functional verification of the selection marker gene. The intermediate recombinant plasmid p15A-CmR-D90-Δ5a/AmpR-ccdB was transformed into two E. coli strains: GBred (CcdB-sensitive) and GBred- gyrA462 (CcdB-resistant). On LB agar plates containing ampicillin and chloramphenicol, only GBred- gyrA462 survived, confirming the toxicity of CcdB and functionality of the selection cassette. In the second recombination step, replacement of the AmpR-ccdB cassette by the target Δ5a/EGFP fragment yielded the final recombinant plasmid p15A-CmR-D90-Δ5a/EGFP. Upon counter-selection with CcdB, only host E. coli GBred harboring the correctly recombined plasmid survived on chloramphenicol-containing LB plates, while non-recombinant bacteria were eliminated.
    Figure Legend Snippet: Validation of recombinant plasmids by restriction enzyme analysis and functional selection screening. (A) Restriction enzyme digestion of intermediate recombinant plasmids was performed using XhoI, XbaI , and KpnI . The observed banding patterns were consistent with the predictions generated by SnapGene software. Lanes 1–3 show the predicted digestion patterns of p15A-CmR-D90, p15A-CmR-D90-Δ5a/AmpR-ccdB, and p15A-CmR-D90-Δ5a/EGFP. Lanes 4–5, 6–7, and 8–9 correspond to the experimental digestion results of these respective plasmids. M: TaKaRa 1 kb DNA Ladder. All digested products were resolved by electrophoresis on 1% agarose gels and visualized with ethidium bromide staining. (B) Functional verification of the selection marker gene. The intermediate recombinant plasmid p15A-CmR-D90-Δ5a/AmpR-ccdB was transformed into two E. coli strains: GBred (CcdB-sensitive) and GBred- gyrA462 (CcdB-resistant). On LB agar plates containing ampicillin and chloramphenicol, only GBred- gyrA462 survived, confirming the toxicity of CcdB and functionality of the selection cassette. In the second recombination step, replacement of the AmpR-ccdB cassette by the target Δ5a/EGFP fragment yielded the final recombinant plasmid p15A-CmR-D90-Δ5a/EGFP. Upon counter-selection with CcdB, only host E. coli GBred harboring the correctly recombined plasmid survived on chloramphenicol-containing LB plates, while non-recombinant bacteria were eliminated.

    Techniques Used: Biomarker Discovery, Recombinant, Functional Assay, Selection, Generated, Software, Electrophoresis, Staining, Marker, Plasmid Preparation, Transformation Assay, Bacteria



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    Validation of recombinant plasmids by restriction enzyme analysis and functional selection screening. (A) Restriction enzyme digestion of intermediate recombinant plasmids was performed using XhoI, XbaI , and KpnI . The observed banding patterns were consistent with the predictions generated by SnapGene software. Lanes 1–3 show the predicted digestion patterns of p15A-CmR-D90, p15A-CmR-D90-Δ5a/AmpR-ccdB, and p15A-CmR-D90-Δ5a/EGFP. Lanes 4–5, 6–7, and 8–9 correspond to the experimental digestion results of these respective plasmids. M: TaKaRa <t>1</t> <t>kb</t> DNA Ladder. All digested products were resolved by electrophoresis on 1% agarose gels and visualized with ethidium bromide staining. (B) Functional verification of the selection marker gene. The intermediate recombinant plasmid p15A-CmR-D90-Δ5a/AmpR-ccdB was transformed into two E. coli strains: GBred (CcdB-sensitive) and GBred- gyrA462 (CcdB-resistant). On LB agar plates containing ampicillin and chloramphenicol, only GBred- gyrA462 survived, confirming the toxicity of CcdB and functionality of the selection cassette. In the second recombination step, replacement of the AmpR-ccdB cassette by the target Δ5a/EGFP fragment yielded the final recombinant plasmid p15A-CmR-D90-Δ5a/EGFP. Upon counter-selection with CcdB, only host E. coli GBred harboring the correctly recombined plasmid survived on chloramphenicol-containing LB plates, while non-recombinant bacteria were eliminated.
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    Validation of recombinant plasmids by restriction enzyme analysis and functional selection screening. (A) Restriction enzyme digestion of intermediate recombinant plasmids was performed using XhoI, XbaI , and KpnI . The observed banding patterns were consistent with the predictions generated by SnapGene software. Lanes 1–3 show the predicted digestion patterns of p15A-CmR-D90, p15A-CmR-D90-Δ5a/AmpR-ccdB, and p15A-CmR-D90-Δ5a/EGFP. Lanes 4–5, 6–7, and 8–9 correspond to the experimental digestion results of these respective plasmids. M: TaKaRa 1 kb DNA Ladder. All digested products were resolved by electrophoresis on 1% agarose gels and visualized with ethidium bromide staining. (B) Functional verification of the selection marker gene. The intermediate recombinant plasmid p15A-CmR-D90-Δ5a/AmpR-ccdB was transformed into two E. coli strains: GBred (CcdB-sensitive) and GBred- gyrA462 (CcdB-resistant). On LB agar plates containing ampicillin and chloramphenicol, only GBred- gyrA462 survived, confirming the toxicity of CcdB and functionality of the selection cassette. In the second recombination step, replacement of the AmpR-ccdB cassette by the target Δ5a/EGFP fragment yielded the final recombinant plasmid p15A-CmR-D90-Δ5a/EGFP. Upon counter-selection with CcdB, only host E. coli GBred harboring the correctly recombined plasmid survived on chloramphenicol-containing LB plates, while non-recombinant bacteria were eliminated.

    Journal: Poultry Science

    Article Title: Construction and modification of a low-copy plasmid-based infectious clone for GI-19 genotype IBV via Red/ET recombineering: A simplified and efficient reverse genetics system for co ronavirus

    doi: 10.1016/j.psj.2026.106881

    Figure Lengend Snippet: Validation of recombinant plasmids by restriction enzyme analysis and functional selection screening. (A) Restriction enzyme digestion of intermediate recombinant plasmids was performed using XhoI, XbaI , and KpnI . The observed banding patterns were consistent with the predictions generated by SnapGene software. Lanes 1–3 show the predicted digestion patterns of p15A-CmR-D90, p15A-CmR-D90-Δ5a/AmpR-ccdB, and p15A-CmR-D90-Δ5a/EGFP. Lanes 4–5, 6–7, and 8–9 correspond to the experimental digestion results of these respective plasmids. M: TaKaRa 1 kb DNA Ladder. All digested products were resolved by electrophoresis on 1% agarose gels and visualized with ethidium bromide staining. (B) Functional verification of the selection marker gene. The intermediate recombinant plasmid p15A-CmR-D90-Δ5a/AmpR-ccdB was transformed into two E. coli strains: GBred (CcdB-sensitive) and GBred- gyrA462 (CcdB-resistant). On LB agar plates containing ampicillin and chloramphenicol, only GBred- gyrA462 survived, confirming the toxicity of CcdB and functionality of the selection cassette. In the second recombination step, replacement of the AmpR-ccdB cassette by the target Δ5a/EGFP fragment yielded the final recombinant plasmid p15A-CmR-D90-Δ5a/EGFP. Upon counter-selection with CcdB, only host E. coli GBred harboring the correctly recombined plasmid survived on chloramphenicol-containing LB plates, while non-recombinant bacteria were eliminated.

    Article Snippet: M: TaKaRa 1 kb DNA Ladder.

    Techniques: Biomarker Discovery, Recombinant, Functional Assay, Selection, Generated, Software, Electrophoresis, Staining, Marker, Plasmid Preparation, Transformation Assay, Bacteria